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Fig. s1
Expression of zebrafish prmt1 mRNA and protein during early development. (A) The expression of prmt1 during different zebrafish developmental stages analyzed by RT-PCR. (B) The expression of prmt1 in different zebrafish adult tissues analyzed by T-PCR. EF indicated the RT-PCR product of elongation factor 1A as a loading control. hpf, hours post fertilization; SP: spleen, S: skin, E: eye, G: gill, H: heart, B: brain, Mu: muscle, O: ovary, T: testis, SB: swim bladder. (C) Endogenous PRMT1 protein (approximately 42 kDa) in extracts of different embryonic stages (1 cell, 6, 24, 48 and 72 hpf) was detected by Western blot analysis. Lane 1: HeLa cell extract was included as a positive control. Detection by anti-β-actin was used as a loading control.