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Fig. 6
Pituitary histology in prop1 MO and control injected embryos observed on plastic sections (A–D; I–L) and by ISH using pitx3 probe mRNA (E–H). (E/F) are ISH on cryosections while (G/H) are whole mounts subsequently sectioned with vibratome. Treatments are indicated in the upper left corner. Gene names and stages are indicated in the lower-right corner of each panel (A–H, hpf; I–L, dpf). Dotted lines delimit detectable pituitary boundaries; blue arrows point to altered morphology. All panels are equally scaled (scale bar = 25 μm).
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