Fig. 7
- ID
- ZDB-IMAGE-110608-6
- Genes
- Antibodies
- Publication
- Chung et al., 2011 - Zebrafish her8a is activated by Su(H)-dependent Notch signaling and is essential for the inhibition of neurogenesis
- All Figures
- Figures for Chung et al., 2011
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Fig. 7 Glial precursors and mature glial cells were reduced in Her8a knockdown embryos.
Her8a morpholino injection downregulates the expression of the glial precursor marker slc1a3a (A, B) and radial glial markers (gfap for radial glial cell body (D, E) and zrf-1 for glial fibers (G, H)), and this effect was rescued by co-injection with her8a mRNA (C, F, I). (A, D, G) Control morpholino, (B, E, H) 8 ng of MO2, (C, F, I) Co-injection of 8 ng of MO2 with 0.5 ng her8a mRNA. Embryos were harvested at the 18-somite stage (A–C) and 36 hpf (D–I). (A–F) in situ hybridization; (G–I) immunohistochemistry with zrf-1 antibody, fluorescent signals were inverted to negative film for a better presentation. (J) qPCR analysis showing the levels of slc1a3a and zash1 and zrf-1 are significantly decreased in embryos injected with her8a morpholino. * p<0.05.