|
Fig. 7 fu Participates in Dorsoventral Patterning by Regulating both Nodal and BMP Signaling Pathways in Zebrafish
(A and B) Embryonic morphology at 24 hpf after downregulating or upregulating Fu activity. Embryos injected with 5 ng fu-MO exhibited more severe necrosis (B) than those injected with 5 ng fu-cMO/p53MO (A).
(C) Coinjection of 5 ng p53MO with 5 ng fu-MO alleviated necrosis as observed in (B) but caused dorsalized phenotypes.
(D) Overexpression of 300 pg fu mRNA led to ventralized phenotypes.
(E–L) Examination of dorsoventral marker genes gata1 (24 hpf) and gsc (shield stage). Compared to control embryos injected with fu-cMO and p53MO (E and I), 5 ng fu-MO injected alone (F and J) or coinjected with 5 ng p53MO (G and K) led to both gata1 inhibition and gsc expansion. A 300 pg fu mRNA injection (H and L) led to an expansion of gata1 and a slight reduction of gsc. Statistical data are shown in (S) and (T). Embryo orientations: lateral views with head to the left for gata1; dorsal views with animal pole to the top for gsc.
(M–R) Compared with the uninjected control (M), embryos injected with 0.75 pg sqt mRNA were classified into D1 and D2 groups of dorsalization (N and O). Embryos injected with 10 pg bmp2b mRNA were classified into V1–V3 groups of ventralization (P, Q, and R).
(U) Statistical data for rescue experiments in which 300 pg fu mRNA was coinjected with 0.75 pg sqt or 10 pg bmp2b mRNA. Coinjection of fu mRNA rescues sqt- or bmp2b-induced dorsoventral patterning defects.
See also Figure S6.
Reprinted from Cell, 143(6), Xia, L., Jia, S., Huang, S., Wang, H., Zhu, Y., Mu, Y., Kan, L., Zheng, W., Wu, D., Li, X., Sun, Q., Meng, A., and Chen, D., The Fused/Smurf Complex Controls the Fate of Drosophila Germline Stem Cells by Generating a Gradient BMP Response, 978-990, Copyright (2010) with permission from Elsevier. Full text @ Cell