ZFIN Image: Kalousova et al., 2010, Fig. 2

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Figure Caption

Fig. 2 Inhibition of DachB expression by morpholino antisense oligonucleotides. Expression of insulin (A–C), somatostatin (D–F) and glucagon (G–I) was analyzed by whole-mount in situ hybridization at 30 hpf embryos injected with the morpholino oligonucleotides shown: a standard control (A, D, G), a mismatch control with 4 bases altered from the complimentary dachb sequence (B, E, H), and an oligonucleotide complimentary to a dachb splice junction (C, F, I). All panels present ventral views of yolk-free embryos with anterior to the left. In panel J, the numbers of cells expressing the hormones shown were assessed in morpholino-injected embryos at 30 hpf. Each data point represents the mean ± standard error of at least 40 embryos for each condition. ***p < 0.001 compared with embryos injected with morpholino 4-mismatch control by Student′s t test. Scale bar, 50 μm.

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Acknowledgments

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Reprinted from Developmental Biology, 348(2), Kalousova, A., Mavropoulos, A., Adams, B.A., Nekrep, N., Li, Z., Krauss, S., Stainier, D.Y., and German, M.S., Dachshund homologues play a conserved role in islet cell development, 143-152, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.