Fig. 4
Loss of zebrafish Gβ1 phenocopies NDPK B morphants. Cardiac phenotype of control morpholino-injected (MO-Ctrl) (A) and MO-Gβ1/Gβ1like double-injected (B) embryos at 72 hpf. Fractional shortening (FS) of the atrial and ventricular chamber of MO-Ctrl fishes (C) and Gβ1/Gβ1like morphants (D) at 48 and 72 hpf. Data are means ± SEM, n = 10–12. Histology sections of a MO-Ctrl (E) and Gβ1/Gβ1like knockdown (F) heart region stained with hematoxylin/eosin. A, atrium, V, ventricle, en, endocardial, my, myocardial layer. (G–J) Whole-mount immunofluorescence following staining with specific antibodies against atrial myosin heavy chain [S46, (G and I)] and entire heart tube myosin heavy chain [MF20, (H and J)] showing unaltered expression of structure proteins.