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Fig. 9 gata2 and gata3 differentially regulate gad67 expression in KA′ and KA′ interneurons. (A-D) Zebrafish embryos injected with mismatch morpholino (A,C) or with morpholinos directed against gata2 mRNA (B,D). gata2 abolishes tal2 expression (B) as well as gad67 expression (D) in the lateral floor plate (arrow). Thus, correct differentiation of KA′ cells in the lateral floor plate requires gata2 function. (E-H) Embryos injected with mismatch morpholino (E,G) or morpholinos directed against gata3 mRNA (F,H). tal2 (E,F) and gad67 (G,H) expression in KA′ cells is abolished by gata3 knockdown (arrowhead), whereas expression in KA′ cells is unaffected (arrow). Embryos were 24 hpf. Anterior left, dorsal up. Scale bar: 25 μm.