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Fig. S4 Spatiotemporal expression of cav-1 during embryogenesis. (A) Whole-mount in situ hybridization was performed with antisense RNA probes in embryos at indicated stages. 1-cell and shield stages, lateral view with the animal pole to the top; Bud stage to 96 hpf, lateral view with the anterior to the top. (B) Relative expression of Cav-1α or -1β during embryogenesis. Embryos at indicated stages were collected for total RNA isolation and subjected to real-time PCR analysis. Expression of 18s ribosomal RNA was used as an internal control. Values are given as mean ± standard deviation, n = 3.
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Reprinted from Developmental Biology, 344(1), Mo, S., Wang, L., Li, Q., Li, J., Li, Y., Thannickal, V.J., and Cui. Z., Caveolin-1 regulates dorsoventral patterning through direct interaction with beta-catenin in zebrafish, 210-223, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.