ZFIN Image: Gates et al., 2010, Fig. S4

Image

Figure Caption

Fig. S4 Rest knockdown enhances the effects of ectopic Hh signaling. Lateral views of embryos injected with control MO (Left column), shh mRNA (middle column) or shh mRNA in conjunction with rest MO (right column). (A-U) Embryos fixed around 24-28hpf and stained for Hh response genes . (V-X) Live 32hpf transgenic islet:eGFP embryos. (Middle column) Injection of shh mRNA results in dorsal expansion (B, E, H, K, N, W) or reduction (Q, T) in displayed markers. (Right column) Co-injection of rest MO alongside shh mRNA causes greater dorsal expansion (C, F, I, L,O, X) and a greater reduction in genes negatively regulated by Hh signaling (R, U).

Acknowledgments

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Reprinted from Developmental Biology, 340(2), Gates, K.P., Mentzer, L., Karlstrom, R.O., and Sirotkin, H.I., The transcriptional repressor REST/NRSF modulates hedgehog signaling, 293-305, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.