ZFIN Image: Chen et al., 2010, Fig. 1

Image

Figure Caption

Fig. 1 Nestin expression in zebrafish embryos.

A–D. Analysis of nestin transcripts by whole-mount in situ hybridization in embryos injected with control MO. A and C, lateral view from left; B, dorsal view, head at top and D, dorsal view, head at left. Scale bar: 200 μm. E. Nucleotide sequence around the translation start site of zebrafish nestin cDNA and corresponding sequence of nestin MO1 and MO2. F. Western blot analysis of nestin:GFP fusion proteins in 30 hpf embryos treated with nestin MO (10 ng) or its 5-mis MO control (10 ng). The proteins were immunoblotted (IB) with a GFP (α-GFP) or actin antibody (α-actin).

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One