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Fig. 1 Phenotypes of scohi459 mutant zebrafish. (A) Side view of embryos at 36 hpf. (B) A mutant at 3 dpf. The arrow points to a cyst. (C,D) Cysts at the glomerular (arrowhead) and tubular (arrow) regions in cross-sections of mutant embryos at 50 hpf. (E-I) Increased size of the pronephric duct in mutant embryos as shown in cross-sections of the duct region (E,F red arrows) at 50 hpf. (G,H) The pronephric duct in side view in whole-mount embryos stained with α6F. (I) Cross sections of pronephric duct at 5 dpf. Anti-Na+/K+-ATPase and anti-Cdh17 are used as basolateral markers. Atypical PKC (aPKC) and rhodamine phalloidin (RhPh) are used as apical markers. DAPI is used as nuclei dye. mut, mutant; nc, notochord; wt, wild type. Scale bars: 10 μm.