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Fig. 5 Zic2a limits distal OS marker expression in the ventral retina. The effect of Zic2a depletion was examined using ISH. (A-D) The following markers were expressed normally in control and zic2a morphants: rx3 in the pre-optic area of the hypothalamus (A, 36/36 control morphants, n=3; B, 42/44 zic2a morphants, n=3); vax1 in the proximal OS (C, 50/50 control morphants, n=4; D, 58/68 zic2a morphants, n=4). (E,F) pax2a is expressed normally in control morphants (E, 51/52 embryos, n=4), and is expanded into the retina in zic2a morphants (F, 123/131 embryos, n=6). (G,H) fgf8a is restricted to the OS in control morphants (G, 20/20 embryos, n=2), and expanded into the retina when Zic2a is depleted (H, 44/53 embryos, n=3). Green arrowheads in F,H point to abnormal expression in the ventral retina. A-H are lateral views, anterior to the left. A′-H′ are ventral views of the same embryos, anterior to the left. All embryos are at prim-5.