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Fig. 8 Adult rapunzel mutants over express skeletal genes during early development. A–F. ISH was performed on sagittal sections from rapunzel mutants and their wild type siblings. A, B. Overexpression (arrows) of col2a1 is seen in 2-week-old rapunzel mutants (A) compared to wild type (B). C–F. Overexpression of col10a1 (C, D) and osx (E, F) is seen in the rostral (arrowheads) and caudal (arrows) craniofacial skeleton, as well as the opercle (double arrowheads) in 4-week-old rapunzel mutants (C, E) compared to wild type (D, F). G–M. Similar results are observed in the fin ray skeleton. Whole-mount ISH reveals overexpression of col2a1 (G, H), col10a1 (I, J), and osx (K, L) in rapunzel fins. These results were confirmed by qPCR (M). N, O. Whole-mount ISH reveals that rpz is diffusely expressed in the head (N, arrow) and vertebral column (O, arrow) of 4-week-old wild type larvae, but does not co-localize with skeletal markers. There were no differences in rpz expression between rapunzel heterozygotes (not shown) and wild type siblings.
Reprinted from Developmental Biology, 334(1), Green, J., Taylor, J.J., Hindes, A., Johnson, S.L., and Goldsmith, M.I., A gain of function mutation causing skeletal overgrowth in the rapunzel mutant, 224-234, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.