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Fig. S9
Laterality defects in cilia knock-down embryos.
In situ hybridization for myocardial marker cmlc2 at 28hpf. In uninjected control embryos, 100% of hearts jogged to the left (A). In 33 – 65% of embryos injected with dnKif3b mRNA, qilin MO1 or qilin MO2, the heart jogged to the right (B) or remained in the midline (C). The table shows the percentage and total number of embryos counted showing left-sided hearts and midline or right-sided hearts for uninjected control embryos and embryos injected with either 200 pg dnKif3b mRNA, 5 ng qilin MO1, or 15ng qilin MO2.
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