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Fig. 3 tbx6 and no tail are transcribed in response to mesoderm induction. Animal caps, removed from 2000- to 4000-cell embryos, were incubated for 5 hr in the absence (Control lane) or the presence of activin or bFGF, provided at the indicated concentrations. RNA was harvested from groups of 10 caps and transcribed genes were detected by RT-PCR. The ker8 (cytokeratin 8) gene is specific to the extraembryonic enveloping layer and serves as a control for the viability of the animal caps and the recovery of RNA.
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Reprinted from Developmental Biology, 183(1), Hug, B., Walter, V., and Grunwald, D.J., tbx6, a brachyury-related gene expressed by ventral mesendodermal precursors in the zebrafish embryo, 61-73, Copyright (1997) with permission from Elsevier. Full text @ Dev. Biol.