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Fig. 3 Gli1, Gli2a, and Gli2b contribute to the Hh response in the pituitary. (A) At 30 hpf nkx2.2a is expressed in the PD (arrowhead) but not in the PI (arrow). lim3 (inset) is expressed in both the PD and PI. (B) In dtr(gli1) mutants, nkx2.2a expression is reduced in the PD (arrowhead) and lim3 expression is reduced, indicating a smaller placode (inset). (C) Injecting gli2a morpholinos (MOs) into wildtype embryos led to a posterior expansion of nkx2.2a and lim3 expression (arrows). (D) Injecting gli2a MOs into dtr(gli1) mutants eliminated nkx2.2a expression in the adenohypophysis, but did not further reduce lim3 expression (inset). (E) Injecting gli2b MOs into wildtype embryos led to a posterior expansion of nkx2.2a and lim3 expression (arrows). (F) gli2b MO injections into dtr(gli1) mutants eliminated nkx2.2a expression in the placode, but did not further reduce lim3 expression (inset). (G) Co-injection of gli2a and gli2b MOs led to posterior expansion of nkx2.2a and lim3 expression (arrows). (H) Co-injection of gli2a and gli2b MOs eliminated nkx2.2a expression and reduced lim3 expression. (I) Dominant repressor forms of Gli2a encoded by the yot locus eliminate nkx2.2a expression in the PD. (J) gli2a MO injections into yot(gli2DR) rescued nkx2.2a expression (arrowhead), with expression resembling that seen in dtr(gli1) mutants (compare to panel B). (A–J) Lateral views of the ventral forebrain, anterior to the left, eyes removed. Scale bar: A–J; 30 μm, insets in panels A–H; 40 μm.
Reprinted from Developmental Biology, 326(1), Devine, C.A., Sbrogna, J.L., Guner, B., Osgood, M., Shen, M.C., and Karlstrom, R.O., A dynamic Gli code interprets Hh signals to regulate induction, patterning, and endocrine cell specification in the zebrafish pituitary, 143-154, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.