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Fig. 3 Ectopic shh reverses or disrupts gene expression in cardiac precursors and visceral precursors. Dorsal views of whole-mounted embryos, anterior to the left. (A) Uninjected wild-type control showing BMP4 expression on the left side of the midline in the heart tube. (B) Embryo injected with shh + GFP mRNA that localized to the right side, as determined by staining with anti-GFP (brown), which is easily visible in the right otic capsule (arrow) and right eye. (C) Uninjected control showing distribution of rtk2 mRNA on the right side of the gut. (D) Reversal in rtk2 expression following injection of shh + GFP on the right side. (E) DM-GRASP in the differentiated heart (arrow) and gut (arrowhead) on the left side. (F) Injected embryo with heart and gut on the right. (G, H) Higher magnification views of reversals in large (arrow) and small (arrowhead) clusters of DM-GRASP expressing cells in the gut. Scale bars, 100 μm.
Reprinted from Developmental Biology, 210(2), Schilling, T.F., Concordet, J.-P., and Ingham, P.W., Regulation of left-right asymmetries in the zebrafish by Shh and BMP4, 277-287, Copyright (1999) with permission from Elsevier. Full text @ Dev. Biol.