Fig. 3 cv2 expression is lost from the PPR, otic placode, and pharyngeal arches in Dlx3b/4b morphants. (A,B) cv2 (purple) is co-expressed with dlx3b (red) in the ectoderm of the PPR. Transverse sections were taken through the neural plate of 3-somite embryos. (C,D) Prior to the onset of dlx3b/4b expression, cv2 expression is unaffected on the ventral side of 60% epiboly embryos. (E,F) cv2 expression is lost from the PPR in Dlx3b/4b morphants (F). (G,H) cv2 expression is lost from the otic placode in 18 somite Dlx3b/4b morphants (H). (I,J) cv2 expression is lost from the otic vesicle and pharyngeal arches in 30 hpf (hours post fertilization) embryos (J). ov, otic vesicle; pa, pharyngeal arches. All views are lateral except (E,F), which are dorsal views.
Reprinted from Developmental Biology, 325(1), Esterberg, R., and Fritz, A., dlx3b/4b are required for the formation of the preplacodal region and otic placode through local modulation of BMP activity, 189-199, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.