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Fig. 4 Mammalian Blimp1 protein can rescue slow myogenesis in zebrafish embryos that lack endogenous Blimp1 activity. (A) A smo embryo showing lack all slow muscle fibers. (B) An ubo embryo with a few residual slow MyHC-positive cells (green, arrow). These cells are not properly differentiated slow muscle fibers, as they do not express Prox1 (red). (C, D) Expression of mammalian Blimp1 from a heat-inducible promoter can rescue slow muscle development in both smo (C) and ubo embryos (D). All panels depict lateral views of 24 hpf embryos stained with mAb F59 (green) and anti-Prox1 (red). Scale bar = 25 μm. The scale bar in A applies to B–D.
Reprinted from Developmental Biology, 324(2), Liew, H.P., Choksi, S.P., Wong, K.N., and Roy, S., Specification of vertebrate slow-twitch muscle fiber fate by the transcriptional regulator Blimp1, 226-235, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.