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Fig. 3 Sizes of the notochord and chordoneural hinge (CNH) are influenced by Bmp4 activity during late gastrulation. (A-E) shha expression in 14-somite stage embryos. Insets are lateral views of the CNH. (F-J) shha expression in sections through somites 5-10 of 18-somite stage embryos. Insets are cropped images of the same section stained with DAPI. The mean number of DAPI-stained cells from 10 experimental embryos was subjected to one-way ANOVA, followed by a two-tailed, equal variance t-test. (G,H,J) Asterisks indicate significance (P<0.001). (K-O) ntl expression in 24 hpf embryos. CNH domains are indicated with arrowheads, arrows indicate to ectopic tail formation. (A-E) Dorsal views, with anterior towards the left. (K-O) Lateral views, with anterior towards the left.