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Fig. S3 Defective gut regionalisation in the vhnf1hi2169 mutant. Whole-mount in situ hybridization in lateral view at 24 hpf. Anterior to the left. krox20 (egr2b) or frb35 (egr2a) staining was used to identify rhombomere 5 (r5), which is strongly reduced in vhnf1hi2169 homozygous embryos. axial (foxa2) and shh are caudally expanded in the mutant gut (B,D) as compared with the wild type (A,C).
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