ZFIN Image: Postel et al., 2008, Fig. S4

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Figure Caption

Fig. S4 Controls for morpholino mediated knock-down of itgα7 and mlp. A,C) RTpcr on cDNA of itgα7-splice MO (A) or mlp-splice MO (B) injected embryos shows severe reduction of the amount of wt transcript compared to uninjected control embryos (uic). As a control for the amount of cDNA, RT-PCR for ef1α was performed. Larger transcript are observed in both the itgα7- splice MO and mlp- splice MO injected embryos (asterisk). Sequencing of these larger PCR product confirmed the defective splicing of both transcripts resulting in a introduction of a premature stop codon. The arrowhead indicates an nonspecific PCR product.
B,D) Genomic sequence of itgα7 exon1-intron1 boundary (B) and mlp exon3-intron3 boundary (D) with MO-target site indicated (in blue) and the premature stop codon present in the intron (in red).

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Expression / Labeling details

Acknowledgments

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Reprinted from Developmental Biology, 318(1), Postel, R., Vakeel, P., Topczewski, J., Knöll, R., and Bakkers, J., Zebrafish integrin-linked kinase is required in skeletal muscles for strengthening the integrin-ECM adhesion complex, 92-101, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.