PUBLICATION
Zebrafish integrin-linked kinase is required in skeletal muscles for strengthening the integrin-ECM adhesion complex
- Authors
- Postel, R., Vakeel, P., Topczewski, J., Knöll, R., and Bakkers, J.
- ID
- ZDB-PUB-080429-10
- Date
- 2008
- Source
- Developmental Biology 318(1): 92-101 (Journal)
- Registered Authors
- Bakkers, Jeroen, Postel, Ruben, Topczewski, Jacek
- Keywords
- Myotendinous junctions, ILK, Integrin, Laminin, loc, Zebrafish, Muscular dystrophy, MLP, β-parvin
- MeSH Terms
-
- Antigens, CD/classification
- Antigens, CD/genetics
- Antigens, CD/metabolism*
- Protein Serine-Threonine Kinases/genetics
- Protein Serine-Threonine Kinases/metabolism*
- Oligonucleotides, Antisense/genetics
- Oligonucleotides, Antisense/metabolism
- Animals
- Phylogeny
- Actinin/genetics
- Actinin/metabolism
- Phenotype
- Two-Hybrid System Techniques
- Cytoskeleton/metabolism
- Zebrafish*/anatomy & histology
- Zebrafish*/embryology
- Zebrafish*/metabolism
- Humans
- Paxillin/genetics
- Paxillin/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Laminin/genetics
- Laminin/metabolism
- Lim Kinases/genetics
- Lim Kinases/metabolism
- Extracellular Matrix/genetics
- Extracellular Matrix/metabolism*
- Muscle, Skeletal*/cytology
- Muscle, Skeletal*/embryology
- Muscle, Skeletal*/metabolism
- Recombinant Fusion Proteins/genetics
- Recombinant Fusion Proteins/metabolism
- Cell Adhesion/physiology*
- Integrin alpha Chains/classification
- Integrin alpha Chains/genetics
- Integrin alpha Chains/metabolism*
- PubMed
- 18436206 Full text @ Dev. Biol.
Citation
Postel, R., Vakeel, P., Topczewski, J., Knöll, R., and Bakkers, J. (2008) Zebrafish integrin-linked kinase is required in skeletal muscles for strengthening the integrin-ECM adhesion complex. Developmental Biology. 318(1):92-101.
Abstract
Mechanical instability of skeletal muscle cells is the major cause of congenital muscular dystrophy. Here we show that the zebrafish lost-contact mutant, that lacks a functional integrin-linked kinase (ilk) gene, suffers from mechanical instability of skeletal muscle fibres. With genetic and morpholino knock-down experiments we demonstrate that: 1) laminin, itgalpha7, Ilk and beta-parvin are all critical for mechanical stability in skeletal muscles. 2) Ilk acts redundantly with the dystrophin/dystroglycan adhesion complex in maintaining mechanical stability of skeletal muscles. 3) Ilk protein is recruited to the myotendinous junctions, which requires the ECM component laminin and the presence of itgalpha7 in the sarcolemma. 4) Ilk, unexpectedly, is dispensable for formation of the adhesion complex. Ilk, however, is required for strengthening the adhesion of the muscle fibre with the ECM and this activity requires the presence of a functional kinase domain in Ilk. 5) We identified a novel interaction between Ilk and the mechanical stretch sensor protein MLP. Thus, Ilk is an essential intracellular component downstream of laminin and itgalpha7, providing strengthening of skeletal muscle fibre adhesion with the ECM and therefore qualified as a novel candidate gene for congenital muscular dystrophy.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping