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Fig. 3 Immunostaining of frozen cross-section of 48 hpf wild type (A) and Col15a1-MO (B) zebrafish embryos using antibodies against mouse collagen XV NC8 domain. (A) In wild type, collagen XV is deposited around the notochord in the peri-notochordal sheath. A labelling is also observed in the skin. (B) Collagen XV immunostaining is completely extinguished in collagen XV morphants attesting for the morpholino efficiency. Note that staining persists in skin. Magnification: x 400.
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Reprinted from Developmental Biology, 316(1), Pagnon-Minot, A., Malbouyres, M., Haftek-Terreau, Z., Kim, H.R., Sasaki, T., Thisse, C., Thisse, B., Ingham, P.W., Ruggiero, F., and Le Guellec, D., Collagen XV, a novel factor in zebrafish notochord differentiation and muscle development, 21-35, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.