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Fig. 2
stra6 and rbp4 mRNA Expression during Zebrafish Development Analyzed by Whole-Mount In Situ Hybridization
(A) At the 12-somite stage, stra6 mRNA (blue) is expressed in the yolk syncytium and in mesendodermal cells in the head and trunk region. Staining is also detectable in the eye anlage, the pineal gland (PG), and anterior somites (arrowheads).
(B) At 24 hours postfertilization (hpf), staining for stra6 (blue) is seen in the developing eyes, the anterior midbrain, the pineal gland (PG), and anterior somites (arrowheads).
(C and D) At the 3 and 4 days postfertilization (dpf) larval stages, stra6 mRNA is expressed in the eyes and the pineal gland.
(E) Cross-section through an eye of a 4 dpf larva shows stra6 expression (blue) in the retinal pigment epithelium (RPE).
(F and G) rbp4 mRNA expression (blue) in a 24 hpf (F) and 48 hpf (G) embryo. Staining patterns reveal that rbp4 is expressed in the yolk syncytial layer. (H) Immunoblot analysis for Rbp4 expression in 15 hpf embryos. Murine plasma (0.1 μl) was used as a control.
Scale bars = 100 μm. Anterior is to the left in all pictures.
Reprinted from Cell Metabolism, 7(3), Isken, A., Golczak, M., Oberhauser, V., Hunzelmann, S., Driever, W., Imanishi, Y., Palczewski, K., and von Lintig, J., RBP4 Disrupts Vitamin A Uptake Homeostasis in a STRA6-Deficient Animal Model for Matthew-Wood Syndrome, 258-268, Copyright (2008) with permission from Elsevier. Full text @ Cell Metab.