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Fig. 3 Cdx4 localizes the pancreas in a dosage-dependent fashion. (A-L) In situ hybridization for insulin expression (purple), photographed in brightfield. A,E,I additionally show krox20 expression in rhombomere 5 and neural crest cells (arrows). (A′-C′,E′-G′,I′-K′) Myosin antibody staining (green fluorescence) to reveal somites. Brightfield and fluorescence images were merged for analysis and are shown separately for clarity. Bracket indicates the somite adjacent to the posterior limit of insulin expression. Arrowheads in I indicate posterior ectopic krox20-positive neural crest cells (out of focus) in 16hpf mutants. The asterisk in K indicates the boundary between somite 6 and 7. Dorsal views, anterior to the left. Magnification: 100x for 16hpf panels; all others 200x.