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Fig. 3 Fine mapping of the 300-bp RetE region. Non-overlapping clusters of point mutations of 20 bp were introduced into -2.4shh:gfpRetE from + 729 to + 1035 of the shh locus (A). The activity of mutant constructs M1–M15 was analyzed by transient expression (B–P). Clustered mutation M12 led to the complete loss of GFP expression (M) whereas mutation M13 abolished GFP expression exclusively in the GCL but not in the INL (N). All other mutant constructs show expression in both layers (B–L, O, P). L, lens; GCL, ganglion cell layer; INL, inner nuclear layer. Scale bar, 50 μm.
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Reprinted from Developmental Biology, 314(1), Vinothkumar, S., Rastegar, S., Takamiya, M., Ertzer, R., and Strähle, U., Sequential and cooperative action of Fgfs and Shh in the zebrafish retina, 200-214, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.