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Fig. 7 MiR-10 acts in synergy with HoxB4 A) Embryos injected with HoxB4, miR-10 siRNA and HoxB4+miR-10 siRNA analyzed for the expression of HoxB1a and HoxB3a at 24 hpf. Injection of 150pg HoxB4 leads to downregulation of HoxB1a and of downregulation of the hindbrain domain of HoxB3a. The rhombomere 4 expression domain of HoxB1a and the rhombomere 5/6 expression domain of HoxB3a are still discernable though. When 150pg HoxB4 is expressed together with miR-10 siRNA the expression domain of HoxB1a disappears and no clear rhombomere 5/6 stripe of HoxB3a expression can be detected. B) Embryos injected with HoxB4, miR-10 siRNA and HoxB4+miR-10 siRNA analyzed for the expression of endogenous HoxB4a at 48 hpf. The combination of HoxB4 together with the miR-10 siRNA induces a stronger phenotype with more severe anterior and posterior truncations than injection with HoxB4 alone. On the right groups of embryos injected with HoxB4 or the combination of HoxB4 and miR-10 siRNA are shown.