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Fig. 4 DFC regional Ca2+ is required for KV morphogenesis. (A) Summary of KV defects. (B-D) Bright field image of wt embryo with normal KV (B) and thapsigargin-treated with reduced (C) and absent (D) KV: dorsal view, 10 somites. Arrows indicate vesicle location; n, notochord. (E-G) charon expression (arrows) around KV in wt (E) and thapsigargin-treated embryos with reduced (F) and absent (G) signal: lateral view, 10 somites. Insets show dorsal view of charon expression. Thap, thapsigargin. Scale bars: 50 μm.
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