Fig. 4 Expression of components of the Nodal-signaling pathway. Sections of wild type embryos at 5 hpf (A) or 4.3 hpf (B–D) embryos stained for cyc (A), oep (B), bon/mixer (C) or foxh1 mRNA (D). (A) In 5 hpf embryos, cyc transcripts are distributed in a punctate pattern in marginal blastomeres within 5 rows of the YSL, and are also detected in the YSL (arrows). (B) oep is expressed in all blastomeres and EVL cells, but transcripts are excluded from the YSL. (C) bon/mixer is expressed exclusively in the blastomeres within 3–4 rows of the YSL. bon/mixer mRNA is not detected in the YSL or EVL. (D) foxh1 transcripts are found throughout the embryo, including all blastomeres and the YSL.
Reprinted from Developmental Biology, 310(2), Fan, X., Hagos, E.G., Xu, B., Sias, C., Kawakami, K., Burdine, R.D., and Dougan, S.T., Nodal signals mediate interactions between the extra-embryonic and embryonic tissues in zebrafish, 363-378, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.