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Fig. 6 Ntl and Tbx16 are not dependent on one another in DFCs. (A–C) Double fluorescent immunostaining of Tbx16 (green) and Ntl (red) in wild-type embryos showed co-expression of these proteins in DFCs (arrowheads) between 70% and 90% epiboly. Tbx16 expression was not detected in tbx16 morphants (D), but Ntl expression in DFCs was unaffected (E). In ntl morphants, Tbx16 was expressed in DFCs (G) in the absence of Ntl protein (H).
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Reprinted from Developmental Biology, 310(2), Amack, J.D., Wang, X., and Yost, H.J., Two T-box genes play independent and cooperative roles to regulate morphogenesis of ciliated Kupffer's vesicle in zebrafish, 196-210, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.