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Fig. 3 Expression of sema3E. Lateral (A,E) and ventral views (C) of whole-mount embryos, and stage-matched transverse sections (B,D,F). A: sema3E label is seen in the posterior tectum at 30 hpf. B,D,F: sema3E transcripts appear in the optic chiasm at 30 hpf and persist until 72 hpf. sema3E is seen in the ventral mesenchymal tissue (B), the hypothalamus (B), and the jaw (F). tec, tectum; hb, hindbrain; H, hypothalamus; j, jaw; oc, optic chiasm; ptec, posterior tectum; vm, ventral mesenchyme. Scale bars in B,D,F = 50 μm.
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