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Fig. 2 Reverse transcription (RT)-PCR detects the continuous presence of transcripts of the two cxcr4s during early development. cxcr4b mRNA is present at a low level during the first 6 h of development. As shown by whole-mount in situ hybridization, its level starts to increase at the early neurula stage (see Fig. 4). cxcr4a transcripts are present at a low level during blastula stage and zygotic transcription is strongly activated at 3.3 hpf. RT-negative control was carried out at the same time and no band was detected (not shown).
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Reprinted from Mechanisms of Development, 109(2), Chong, S.-W., Emelyanov, A., Gong, Z., and Korzh, V., Expression pattern of two zebrafish genes, cxcr4a and cxcr4b, 347-354, Copyright (2001) with permission from Elsevier. Full text @ Mech. Dev.