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Fig. S3 RT-PCR on Jagged mRNAs. A; RT-PCR on embryos injected with jag1spliMO and jag2spliMO and controls. M = molecular weight marker, 1 = embryos injected with splicing-morpholino, 2 = embryos injected with mismatched morpholino, 3 = non-injected controls. B; RT-PCR of jagged genes on embryos at different stages of development. M = molecular weight marker, 1 = 1–2 cell stage, 2 = high stage, 3 = 50% epiboly stage, 4 = 80% epiboly stage, 5 = 5 somite stage, 6 = 16–18 hpf, 7 = 24 hpf, 8 = adult, - = negative control.
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Reprinted from Developmental Biology, 301(1), Zecchin, E., Filippi, A., Biemar, F., Tiso, N., Pauls, S., Ellertsdottir, E., Gnugge, L., Bortolussi, M., Driever, W., and Argenton, F., Distinct delta and jagged genes control sequential segregation of pancreatic cell types from precursor pools in zebrafish, 192-204, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.