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Fig. 5 Zangptl2 expression in adult fin regeneration. Whole-mount in situ hybridization with a Zangptl2 probe was performed on regenerated fins at 0hpa (A), 12hpa (B), 24hpa (C), 48hpa (D) and 96hpa (E). (F) A longitudinal section of a stained fin shows Zangptl2 expression in the blastema under the regenerated epidermis. (G) A longitudinal section of a fin stained with msxb as a marker of blastema is shown. (H) RT-PCR analysis of regenerated fins revealed induction of Zangptl2 expression during the course of fin regeneration. Fins stained with shh as known to be expressed in basal layer of the epidermis in proximity to blastema at 48hpf (I) and 96hpf (J) are shown. Experiments were also performed in water containing DMSO (K) or 10 μM of the Fgfr inhibitor SU5402 (fgfRi) (L,M), and stained with Zangptl2. Zangptl2 expression was unchanged following treatment with DMSO alone at 24hpa (K), but was not detected when fish were treated with SU5402 at 24hpa (L) and 48hpa (M). Closed arrowheads, blastema; open arrowheads, basal layer of the epidermis in close proximity to blastema. Bars indicate 50 μm.
Reprinted from Gene expression patterns : GEP, 5(5), Kubota, Y., Oike, Y., Satoh, S., Tabata, Y., Niikura, Y., Morisada, T., Akao, M., Urano, T., Ito, Y., Miyamoto, T., Watanabe, S., and Suda, T., Isolation and expression patterns of genes for three angiopoietin-like proteins, Angptl1, 2 and 6 in zebrafish, 679-685, Copyright (2005) with permission from Elsevier. Full text @ Gene Expr. Patterns