Fig. 6 oep function is not required before 3 hpf. Images of uninjected MZoep mutants (A–C), or those injected with 100 pg sOep mRNA into the YSL at 3 h (D–F) or 4 h (G–I). MZoep embryos lack all derivates of mesoderm and endoderm in the head and trunk at 24 h (A), and lack expression of cyc (B) and sqt (C) at 5 hpf. Injection of sOep mRNA at 3 hpf restores mesoderm and endoderm (D), as well as cyc (E) and sqt (F) expression. Embryos injected at 4 h are severely cyclopic, but contain trunk somites and notochord (G). Expressions of both cyc (H) and sqt (I) are severely reduced. Animal pole views, with dorsal to the right.
Reprinted from Developmental Biology, 309(2), Hagos, E.G., Fan, X., and Dougan, S.T., The role of maternal Activin-like signals in zebrafish embryos, 245-258, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.