ZFIN Image: Mao et al., 2006, Fig. 1

Image

Figure Caption

Fig. 1 Detection of Hsp27 and alpha-B crystallin mRNA in zebrafish embryonic and adult tissues. (A) RNA was obtained from at least 10 embryos at indicated times post fertilization and RT–PCR conducted using primers specific for Hsp27 and Ef1α mRNA (A′). The amount of starting template was adjusted to produce equal detection of ef1α. (B) Equal amounts of RNA (140 ng) obtained from embryos at 18 hpf (lane 1) and adult skeletal muscle (lane 2) analyzed for hsp27 expression. (C) Conditions and samples used to detect Hsp27 mRNA were used to examine expression of alpha-B crystallin mRNA. (D) Detection of EF1α (lane 1) and alpha-B crystallin mRNA (lane 2) in adult zebrafish lens RNA by RT–PCR. Arrows show the position of a 3Kb MW marker.

Figure Data

Expression / Labeling details

Acknowledgments

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Reprinted from Gene expression patterns : GEP, 6(2), Mao, L., and Shelden, E.A., Developmentally regulated gene expression of the small heat shock protein Hsp27 in zebrafish embryos, 127-133, Copyright (2006) with permission from Elsevier. Full text @ Gene Expr. Patterns