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Fig. 6 Effects of Heat Shock in hsp70:HA-her7 Transgenics. (A) Levels of her7 mRNA expression as shown by ISH (NBT/BCIP) in embryos fixed immediately after a 37 °C heat shock for the indicated numbers of minutes. (B) Levels of her7 mRNA in embryos given a 15-min heat shock followed by 25, 45, or 75 minutes of recovery; almost all her7 mRNA, including the product of the normal endogenous gene, has disappeared at the 15+25-min time point, implying that Her7 inhibits her7 expression. (C) Levels of HA-tagged Her7 protein, revealed by immunostaining with an anti-HA antibody, in embryos given a 30-min heat shock followed by 10, 30, or 60 min of recovery. (D) Effects on expression of her1, deltaC, and deltaD in embryos fixed immediately after a 40-min heat shock. hsp70:HA-her7 transgenics are shown on the left, wild-type sibling controls on the right. All specimens in this figure have been dissected off the yolk and flat mounted.