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Fig. S3 Epicardial Expression of fgfrs during Heart Regeneration. (A) At 3 dpa, fgfr2 and fgfr4 are expressed in contiguous domains of atrial epicardial cells (violet stains,arrowheads), distant from the injured ventricular apex (not shown). (B) Double in situ hybridization colocalizing fgfr4 (red) and tbx18 (green) at 14 dpa, demonstrating fgfr4 expression in epicardial-derived cells. fgfr4 was expressed in the tbx18-positive cells closest to the apical edge of the regenerate (boxes). Scale bar = 100 μm.
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Reprinted from Cell, 127(3), Lepilina, A., Coon, A.N., Kikuchi, K., Holdway, J.E., Roberts, R.W., Burns, C.G., and Poss, K.D., A Dynamic Epicardial Injury Response Supports Progenitor Cell Activity during Zebrafish Heart Regeneration, 607-619, Copyright (2006) with permission from Elsevier. Full text @ Cell