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Fig. 11 Knockdown of Robo3var2 causes expansion of krox20 and papc expression domains. Dorsal views (anterior to the top) of krox20 and papc RNA in situ hybridization at 11 hpf. Six to 9 ng of robo3var2 4 mm control or robo3var2 MO was injected into each embryo. Control embryos did not exhibit any significant defects in the width (asterisks) of krox20 or papc domains (A, C) but robo3var2 MO injected embryos exhibited expansion of both of these expression domains (B, D).
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Reprinted from Mechanisms of Development, 122(10), Challa, A.K., McWhorter, M.L., Wang, C., Seeger, M.A., and Beattie, C.E., Robo3 isoforms have distinct roles during zebrafish development, 1073-1086, Copyright (2005) with permission from Elsevier. Full text @ Mech. Dev.