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Fig. S3 Effects of RA on cyp26b1 and cyp26c1 expression. RNA in situs at the 3-somite stage showing cyp26bc1 (A-C) and cyp26b1 expressions (D-F). In the presence of 100 nM RA, posterior rhombomeres, including r4, are expanded anteriorly, and the r4 expression of cyp26b1 and cyp26c1 are correspondingly expanded (B,E). In the presence of 10 μM DEAB, which blocks RA synthesis, anterior hindbrain regions, including r2 and r3, are expanded posteriorly, and the r2 and r3 domains of cyp26b1 and cyp26c1 are correspondingly expanded (C,F). Thus, neither gene requires RA for its onset in the hindbrain, although both genes are affected by treatments that alter RA-dependent hindbrain patterning events.