|
Fig. 9 Agarose Gel Electrophoresis of products of amplification of adult organ cDNA with exon-specific primers. Total RNA was extracted from pooled adult zebrafish brains and hearts as described in methods. Aliquots of the RNA were converted to cDNA and then amplified with the indicated exon-specific and β-actin primers. Aliquots of 5′RACE clone plasmid DNA corresponding to each exon (except exon 1d for which c241 served as template) were also amplified. The amplification products were subjected to electrophoresis through a 1.5% agarose gel, stained with ethidium bromide, and photographed under UV light using a digital camera.