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Fig. 7 Defects in ectodermal specification and survival in low mutants and in larvae injected with ap2bMO. (A-I) TUNEL labeling of apoptotic cells at 28 hpf is shown in lateral views of the head (A-F) and tail (G-I). (D-F) Co-labeling with TUNEL and the fli1-GFP transgene showing apoptosis lateral to the NCCs in the surface ectoderm (arrows). (J-L) In situ hybridization for nkx2.3 at 28 hpf in dorsal view. Expression is detected in pharyngeal ectoderm and endodermal pouches (arrowheads). b, branchial; h, hyoid; m, mandibular; mf, median fin; n, notochord; ot, otic vesicle; sc, spinal cord. Scale bars: 100 µm.