Fig. 4 Rhombomeric patterning and differentiation of the neurons are unaffected in the llk embryo. (A,B) hoxb1a; (C,D) krox20; (E,F) valentino/mafB mRNA expression in the wild-type (A,C,E) and llk rw16 (B,D,F) embryos at 20 hpf. Expression patterns of these genes are unaffected in the llk rw16 embryo. Dorsal views. The position of the ears are indicated with broken lines. (G,H) Commissural axons are labeled with zn-5 antibody (red) in the wild-type (G) and llk rw16 (H) embryos at 36 hpf. (I,J) Reticulospinal neurons are retrogradely labeled (red) in the wild-type (I) and llk rw16 (J) embryos at 5 dpf. Single-channel images of the labeled neurons are shown in separate panels (I′,J′). M, Mauthner′s cell. Asterisks indicate r6-derived putative OLe neurons. Arrowheads indicate the facial motor nuclei. Dorsal views. Scale bar: 50 µm.
                
                    
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