Gene
nos1
- ID
- ZDB-GENE-001101-1
- Name
- nitric oxide synthase 1 (neuronal)
- Symbol
- nos1 Nomenclature History
- Previous Names
-
- nNOS
- Type
- protein_coding_gene
- Location
- Chr: 5 Mapping Details/Browsers
- Description
- Predicted to enable FMN binding activity; flavin adenine dinucleotide binding activity; and nitric-oxide synthase activity. Acts upstream of or within several processes, including hematopoietic stem cell differentiation; nervous system development; and nitric oxide mediated signal transduction. Predicted to be located in dendritic spine and sarcolemma. Predicted to be active in several cellular components, including cytosol; nucleus; and postsynaptic density. Is expressed in several structures, including brain; enteric nervous system; nitrergic neurons; ovary; and spinal cord. Human ortholog(s) of this gene implicated in Alzheimer's disease; Parkinson's disease; asthma; and cystic fibrosis. Orthologous to human NOS1 (nitric oxide synthase 1).
- Genome Resources
- Note
- None
- Comparative Information
-
- All Expression Data
- 19 figures from 15 publications
- Cross-Species Comparison
- High Throughput Data
- Thisse Expression Data
- No data available
Wild Type Expression Summary
- All Phenotype Data
- 10 figures from 4 publications
- Cross-Species Comparison
- Alliance
Phenotype Summary
Mutations
| Allele | Type | Localization | Consequence | Mutagen | Supplier |
|---|---|---|---|---|---|
| la011122Tg | Transgenic insertion | Unknown | Unknown | DNA | |
| sa11824 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa12337 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa14542 | Allele with one point mutation | Unknown | Splice Site | ENU | |
| sa20353 | Allele with one point mutation | Unknown | Splice Site | ENU | |
| sa26415 | Allele with one point mutation | Unknown | Splice Site | ENU | |
| sa33543 | Allele with one point mutation | Unknown | Splice Site | ENU | |
| sh336 | Allele with one delins | Unknown | Unknown | TALEN |
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| Targeting Reagent | Created Alleles | Citations |
|---|---|---|
| CRISPR1-nos1 | Yu et al., 2024 | |
| CRISPR2-nos1 | Yu et al., 2024 | |
| CRISPR3-nos1 | Moreno-Campos et al., 2024 | |
| MO1-nos1 | N/A | (4) |
| MO2-nos1 | N/A | (4) |
| TALEN1-nos1 | Gutiérrez et al., 2017 |
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Human Disease
Domain, Family, and Site Summary
| Type | InterPro ID | Name |
|---|---|---|
| Domain | IPR001094 | Flavodoxin-like |
| Domain | IPR001433 | Oxidoreductase FAD/NAD(P)-binding |
| Domain | IPR001478 | PDZ domain |
| Domain | IPR001709 | Flavoprotein pyridine nucleotide cytochrome reductase |
| Domain | IPR003097 | Sulfite reductase [NADPH] flavoprotein alpha-component-like, FAD-binding |
| Domain | IPR004030 | Nitric oxide synthase, N-terminal |
| Domain | IPR008254 | Flavodoxin/nitric oxide synthase |
| Domain | IPR017927 | FAD-binding domain, ferredoxin reductase-type |
| Family | IPR012144 | Nitric-oxide synthase, eukaryote |
| Family | IPR050607 | Nitric Oxide Synthase (NOS) |
| Homologous_superfamily | IPR017938 | Riboflavin synthase-like beta-barrel |
| Homologous_superfamily | IPR023173 | NADPH-cytochrome p450 reductase, FAD-binding, alpha-helical domain superfamily |
| Homologous_superfamily | IPR029039 | Flavoprotein-like superfamily |
| Homologous_superfamily | IPR036034 | PDZ superfamily |
| Homologous_superfamily | IPR036119 | Nitric oxide synthase, N-terminal domain superfamily |
| Homologous_superfamily | IPR039261 | Ferredoxin-NADP reductase (FNR), nucleotide-binding domain |
| Homologous_superfamily | IPR044940 | Nitric oxide synthase, domain 2 superfamily |
| Homologous_superfamily | IPR044943 | Nitric oxide synthase, domain 1 superfamily |
| Homologous_superfamily | IPR044944 | Nitric oxide synthase, domain 3 superfamily |
Domain Details Per Protein
| Protein | Additional Resources | Length | FAD-binding domain, ferredoxin reductase-type | Ferredoxin-NADP reductase (FNR), nucleotide-binding domain | Flavodoxin-like | Flavodoxin/nitric oxide synthase | Flavoprotein-like superfamily | Flavoprotein pyridine nucleotide cytochrome reductase | NADPH-cytochrome p450 reductase, FAD-binding, alpha-helical domain superfamily | Nitric oxide synthase, domain 1 superfamily | Nitric oxide synthase, domain 2 superfamily | Nitric oxide synthase, domain 3 superfamily | Nitric-oxide synthase, eukaryote | Nitric Oxide Synthase (NOS) | Nitric oxide synthase, N-terminal | Nitric oxide synthase, N-terminal domain superfamily | Oxidoreductase FAD/NAD(P)-binding | PDZ domain | PDZ superfamily | Riboflavin synthase-like beta-barrel | Sulfite reductase [NADPH] flavoprotein alpha-component-like, FAD-binding |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| UniProtKB:Q800V0 | InterPro | 1431 | |||||||||||||||||||
| UniProtKB:A0A0R4IQG7 | InterPro | 1431 |
Interactions and Pathways
No data available
Plasmids
No data available
No data available
| Relationship | Marker Type | Marker | Accession Numbers | Citations |
|---|---|---|---|---|
| Contained in | BAC | CH211-244P16 | ZFIN Curated Data | |
| Contained in | BAC | DKEY-60F6 | ZFIN Curated Data |
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| Type | Accession # | Sequence | Length (nt/aa) | Analysis |
|---|---|---|---|---|
| RNA | RefSeq:NM_131660 (1) | 4552 nt | ||
| Genomic | GenBank:CR735143 (2) | 147626 nt | ||
| Polypeptide | UniProtKB:A0A0R4IQG7 (1) | 1431 aa |
- Zebrafish Nomenclature Committee (2025) Nomenclature Data Curation (2025). Nomenclature Committee Submission.
- Moreno-Campos, R., Singleton, E.W., Uribe, R.A. (2024) A targeted CRISPR-Cas9 mediated F0 screen identifies genes involved in establishment of the enteric nervous system. PLoS One. 19:e0303914e0303914
- Yu, C., Li, X., Ma, J., Liang, S., Zhao, Y., Li, Q., Zhang, R. (2024) Spatiotemporal modulation of nitric oxide and Notch signaling by hemodynamic-responsive Trpv4 is essential for ventricle regeneration. Cellular and molecular life sciences : CMLS. 81:6060
- Chen, F., Köhler, M., Cucun, G., Takamiya, M., Kizil, C., Cosacak, M.I., Rastegar, S. (2023) sox1a:eGFP transgenic line and single-cell transcriptomics reveal the origin of zebrafish intraspinal serotonergic neurons. iScience. 26:107342107342
- Kuil, L.E., Kakiailatu, N.J.M., Windster, J.D., Bindels, E., Zink, J.T.M., van der Zee, G., Hofstra, R.M.W., Shepherd, I.T., Melotte, V., Alves, M.M. (2023) Unbiased characterization of the larval zebrafish enteric nervous system at a single cell transcriptomic level. iScience. 26:107070107070
- Annona, G., Ferran, J.L., De Luca, P., Conte, I., Postlethwait, J.H., D'Aniello, S. (2022) Expression Pattern of nos1 in the Developing Nervous System of Ray-Finned Fish. Genes. 13(5)
- Banu, S., Gaur, N., Nair, S., Ravikrishnan, T., Khan, S., Mani, S., Bharathi, S., Mandal, K., Kuram, N.A., Vuppaladadium, S., Ravi, R., Murthy, C.L.N., Quoseena, M., Babu, N.S., Idris, M.M. (2022) Transcriptomic and proteomic analysis of epimorphic regeneration in zebrafish caudal fin tissue. Genomics. 114(2):110300
- Capon, S.J., Uribe, V., Dominado, N., Ehrlich, O., Smith, K.A. (2022) Endocardial identity is established during early somitogenesis by Bmp signalling acting upstream of npas4l and etv2. Development (Cambridge, England). 149(9):
- Deng, Y., Wang, L., Wei, T., Chen, Y., Wu, X., Guo, Y., Lin, H., Tang, H., Liu, X. (2022) Inhibition of oocyte maturation by nitric oxide synthase 1 (NOS1) in zebrafish. General and comparative endocrinology. 321-322:114012
- Hariom, S.K., Nelson, E.J.R. (2022) Effects of short-term hypergravity on hematopoiesis and vasculogenesis in embryonic zebrafish. Life sciences in space research. 34:21-29
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