Fig 4

Adult yap1^kg151 have kyphoscoliosis and/or smaller vertebrae.
Heterozygous in-crosses of yap1^kg151/+ reared at the permissive 20.5ºC temperature (A,C-H) or wwtr1^kg169/+ reared to 28.5ºC (B) until 5 dpf-equivalent and then at 26.5ºC to the indicated age, points plotted with jitter to avoid overlap. (A) Reduced growth of yap1^kg151 mutants. Some yap1^kg151 mutant individuals showed a dramatic tail truncation not observed in non-mutant (inset). (B) At 3 months, adult wwtr1^kg169 mutants have similar weight and length to their wild type and heterozygous siblings but survive poorly (Χ², n = 165, p = 0.02). (C) Computerised Tomography (CT) scans of adult fish at 4.5 months viewed from lateral and dorsal. (D) NADH tetrazolium reductase stain showing comparable oxidative/slow (arrows), intermediate (arrowheads) and glycolytic/fast fibres (asterisks) between wild type and yap1^kg151 mutant muscle in superficial (left) and medial (right) myotome transverse cryosections taken at 6 months. (E) Lateral view of wholemount alcian red stained 5.5 month old skeletons. (F) Frequency of curved spine within sibling 4.5-5.5 month adult fish is greater in surviving mutants from four separate yap1^kg151/+ in-crosses compared to their siblings (Χ², n = 340, p = 1 x 10^-11). (G) Spine length of 5.5 month sibling wt and yap1^kg151 fish. (H) Caudal vertebrae length, height and posterior height of 5.5 month straight and curved yap1^kg151 fish. Statistics show significant results of Kruskal-Wallis tests with Dunn’s post hoc comparisons (G,H). Bars: A,C and E = 5 mm; D = 100 μm. Number of fish analysed is indicated on columns.