Fig 3

Yap1^kg151 mutants are defective in larval movement.
Muscle properties analysed in yap1 mutants and their non-mutant siblings on the ß-Actin:membEGFP background reared at the indicated temperature from 70% epiboly until prim 10 (B, see schematic) or 5 dpf-equivalent (A,C-E) and 26.5ºC thereafter (E). (A)MZyap1^kg137 or MZyap1^kg137/kg152 mutants and their heterozygote siblings grown at non-permissive temperature have similar muscle growth. Lack of signal in occasional fibres due to mosaic transgene expression (asterisks) is distinguished from broad loss of fluorescence (#) in oedematous MZ mutants at 5 dpf. Bar = 50 μm. (B)Yap1^kg151 mutants grown as schematised had myotome volume indistinguishable from their heterozygous and wt siblings. (C-E)Yap1^kg151/+ in-cross grown at permissive temperature from 70% epiboly. Symbol shape indicates lay. Bar = 500 μm. (C)Yap1^kg151 mutants at 6 days (equivalent to ~4.25 dpf) swim away from touch stimulation slower than their siblings (upper panel), even when corrected for shorter total length (lower panel). (D) When stimulated by electrical pulses, yap1^kg151 mutants at 7 days (equivalent to 5 dpf) move their trunk through a smaller angle in comparison to their siblings. (E) Swimming velocity of yap1^kg151 mutants at 13 days (equivalent to 11 dpf) is variable between lays. Symbol shapes represent different lays/experiments (3 lays C, 2 lays D,E). Number of fish analysed is indicated on columns. Significant statistical results are shown (A-E) for Kruskal-Wallis tests with Dunn’s post hoc comparisons.