Fig. 2

Lipid dyslipidemia and transport in chd7^+/+ and chd7^−/− mutants. A: Representative images for 3 independent experiments (n = 15 larvae per replicate and genotype) of neutral lipid ORO staining in 3 dpf chd7^+/+ (top) and chd7^−/− mutants (bottom). B: Representative images for 3 independent experiments (N) of ORO staining in 5 dpf chd7^+/+ (top) and chd7^−/− mutants (bottom) with close up of caudal vein region (chd7^+/+; N = 3, n = 12 and chd7^−/−; N = 3, n = 11). C: Representative imaging for 3 independent experiments of BODIPY FL-C12 fluorescence at 3 dpf and 3 hpi chd7^+/+ (n = 6 larvae per experiment) and chd7^−/− mutants (n = 5 larvae per experiment) with overview and close up of vascular system. D: Quantitative analysis of BODIPY signal. Ratio of average intensity measured in the yolk against intensity in the vasculature. (N = 3, n = 5 larvae per experiment). E: Imaging of BODIPY FL-C12 fluorescence at 5 dpf and 48 hpi chd7^+/+ (n = 5 larvae per experiment) and chd7^−/− mutants (n = 4 larvae per experiment) and close up of vascular system. Representative images for 3 independent experiments (n represents larvae per sample/genotype). F: Quantitative analysis of BODIPY signal. Ratio of fluorescence intensity measured in the yolk against swim bladder. (N = 3, n = 4–5 larvae per experiment). (Significance: ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001, Error bars represent SD). Images were taken using a Zeiss Apotome.