FIGURE

Fig 6

ID

ZDB-FIG-260613-16

Publication

Lubash et al., 2026 - RNA Polymerase III subunit Polr3a is required for craniofacial cartilage and bone development in zebrafish

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Fig 6

Pol III-mediated transcription and ribosome biogenesis are diminished in polr3a^sa907/sa907 zebrafish.
(A) Pol III transcribes a variety of non-coding RNAs transcribed by three different types of promoters. Examples of genes transcribed by Pol III are represented. Created in BioRender. Watt, K. (2026) https://BioRender.com/qbgot2s(B) Analysis of total 5S rRNA and tRNAs on the Agilent Bioanalyzer at 5 dpf shows significant reductions in polr3a^sa907/sa907 zebrafish. **p = 0.003; *p = 0.035. (C) qRT-PCR for pre-tRNAs and 7SL RNA at 3 dpf reveals consistent downregulation across all genes examined. ****p < 0.0001. (D) Polysome profiling at 5 dpf reveals diminished ribosome assembly, showing a reduction in the 80S peak and a trend of smaller polysome peaks. (E) At 3 dpf, polr3a^sa907/sa907 zebrafish show diminished 5’ETS expression, a region of the 47S rRNA transcribed by Pol I. ****p < 0.0001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	polr3a^sa907/sa907 (AB)
Observed In:	whole organism 5S class rRNA transcription by RNA polymerase III whole organism ribosome biogenesis whole organism tRNA transcription by RNA polymerase III
Stage:	Day 5

Phenotype Detail

Acknowledgments

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