FIGURE 4

Estimation of bacterial species associated with tet‐resistance genes detected from aquaculture environments. The tetracycline resistance genes sequences identified by comprehensive sequence analysis in Figure 3 were mapped to known tetracycline resistance gene sequences, and the consensus sequences corded in the obtained sequences data were determined. Using these consensus sequences, BLASTn searches were performed against the type sequence materials in the core_n database to identify bacterial species with genes showing ≥ 95% sequence similarity to these consensus sequences. The 16S rRNA gene sequences of these identified bacterial species were retrieved and used as references to analyse the proportion of 16S rRNA gene sequences associated with these bacterial species within the 16S rRNA gene sequences obtained from environmental DNA. Bacterial species with at least one detected read were listed, and their proportions in the bacterial flora were visualised in a heatmap, linking them to their associated tet‐resistance genes. Bacterial species with detection frequencies of ≥ 0.02% in any sample are highlighted in red.